The innuSPEED Bacteria/Fungi RNA Kit has been designed for rapid homogenization and includes an efficient process for digesting the cell walls of fungal spores and gram+ bacteria in specially developed Lysis Tubes; the process utilizes the SpeedMill (or other homogenizers). The starting sample is then denatured; the unique Lysis Buffer deactivates endogenous and exogenous RNases. Once the genomic DNA has been selectively removed, the RNA is bound to the surfaceof a Spin Filter membrane, washed and then finally eluted from the membrane. The isolated RNA is immediately available for downstream applications.
Features
- Extraction of total cellular RNA from fungal spores and gram+ bacteria
- No DNase I digestion: filter membrane for fast, selective removal of genomic DNA
- Integrated inactivation of endogenous and exogenous RNases
- Improved extraction without the use of toxic β-mercaptoethanol