The innuSPEED Tissue RNA Kit allows users to isolate total RNA from a variety of tissue samples quickly and efficiently through the use of a homogenizer. A SpeedMill (or other homogenizer) is used as an effective means of digesting the starting material within the Lysis Tubes contained in the kit. Lysis is performed without the use of highly toxic β-mercaptoethanol, after which the genomic DNA is removed through an initial Spin Filter column. The RNA is then bound to a second Spin Filter membrane, washed and finally eluted in 30 - 80 µl of RNase-free water.
Features
- Isolation of total cellular RNA from a variety of tissues
- Kit contains Lysis Tubes with beads for homogenization
- Optimized for using the SpeedMill
- May also be used with other homogenizers
- Improved extraction without the use of toxic β-mercaptoethanol