- For use following cell lysis measures including lysis reagents, grinding via micropestle, and shearing via passing the lysate through a 20-G needle
- Designed to filter out the excess tissue materials and contaminants prior to the RNA binding step
IBI replacement MINI Total RNA Filter Columns and Collection Tubes have been designed specifically for purifying Total RNA from tissue or plant samples. These columns can be used to utilize excess reagents from other RNA purification products that also use a lysis method. Protocols from the original, complete kit (regardless of brand) may be used with these columns as well. (Note: These replacement columns are NOT RNA-binding columns.)
The RNA Filter Columns and Collection Tubes are a direct replacement for the RNA Filter Columns in the following IBI Total RNA Kits: MINI Total RNA Kit for Tissue (B003S83, B003S84, B003S85, C987R34) and MINI Total RNA Kit for Plant B003S88, B003S89, B003S90).
Sample Size: Up to 25mg of tissue, 25mg paraffin-embedded tissue, 100mg fresh plant tissue, 25mg dry plant tissue
Format: Spin column
Operation: Centrifuge
Expected yield: 5 – 30µg (from tissue); 15 – 20µg of RNA (from 50mg Arabidopsis thaliana leaf)
Elution volume: 25 – 100 µl
Operation time: within 15 minutes, depending on sample type