Zn-immobilized metal affinity chromatography (IMAC) columns consist of Zn-Chelated Separopore®matrix conveniently prepacked spin columns for gravity flow chromatography. Zn-IMAC Columns separate proteins via their differences in affinity to Zn2+ metal ions. Ideal for purification of recombinant proteins containing (His)6 fusion tag.
- Imminodiacetate immobilized matrix charged with Zn metal ions.
- Chelating group coupling method: epoxy activation
- Matrix: cross-linked Separopore® 4B
- Particle size (range): 50-170µm
- Metal ion capacity: > 20µmol Zn2+/ml gel
- Spacer arm: 14 atom
- pH stability: 3-12
- Binding Capacity: Approx. 40 mg His-tagged protein/ml medium
- Supplied as suspension in 20% ethanol.