Anti-GFP Tag (GF28R) mAb recognizes both the native and denatured forms of GFP (Green Fluorescent Protein) and its many popular variants: EGFP, YFP, EYFP, and CFP.
Description: Anti-GFP (Green Fluorescent Protein) Tag (GF28R) Monoclonal Antibody
Clonality: GF28R
Host Species: Mouse
Isotype: IgG1
Immunogen: GFP from the jellyfish Aequorea Victoria N-terminal peptide-KLH conjugates
Specificity: Recognizes native and denatured forms of GFP and its variants such as: EGFP, YFP, EYFP, and CFPM
Purification: Protein A affinity chromatography from mouse ascites fluid
Applications: Dot, ELISA, IP, IS, WB (see notations)
Sensitivity: WB (with ECL): 1-10 ng of purified GFP or GFP fusion proteins
Form: 1mg/mL in 10mM PBS, pH 7.2
Working Conditions: WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature)
IS: 1:500-2000 dilution
For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test
Storage: Store at 4°C (add 0.1% NaN3) for several days to weeks, and at -20°C for longer periods. Avoid repeated freeze-thaw cycles
Product Type : Tag Abs / Tag Antibodies / Tag Antibody