Co-Chelated Separopore® is an imminodiacetate (IDA) immobilized metal affinity chromatography (IMAC) matrix that seperates proteins via their differences in affinity to nickel metal ions.
Properties
- Affinity matrix for proteins.
- Imminodiacetate immobilized matrix charged with Co metal ions.
- Ideal for purification of recombinant proteins containing (His)6 fusion tag.
- Supplied as suspension in 20% ethanol.
- Chelating group: Imminodiacetic acid.
- Chelating group coupling method: epoxy activation.
- Matrix: cross-linked Separopore®, 4% beads.
- Particle size (range): 50-175µm.
- Metal ion capacity: > 20µmol Co2+/ml gel.
- Spacer arm: 14 atom.
- pH stability: 3-12.
- Chem. Stability 40°C for 7 days in: 0.01 M HCl, 1 M NaOH, acetate buffer pH 4,20% ethanol.
- Autoclavable At 121°C, 20% ethanol.
- Antimicrobial agent 20% ethanol.
- Chelating Gp. Coup. Linkage Ether.
Usage : bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemica