Ni-Chelated Separopore® 4B is an imminodiacetate (IDA)-immobilized metal affinity chromatography (IMAC) matrix that seperates proteins via their differences in affinity to nickel metal ions.
Properties
- Imminodiacetate immobilized matrix charged with Ni metal ions.
- IIdeal for purification of recombinant proteins containing (His)6 fusion tag.
Technical Specifications
- Chelating group: Imminodiacetic acid
- Chelating group coupling method: epoxy activation
- Matrix: Cross-linked Separopore® 4% agarose beads
- Particle size (range): 50-168µm
- Metal ion capacity: > 20µmol Ni2+/ml gel
- pH stability: 2-11
- Binding Capacity: Approx. 5-8 mg histidine-tagged protein/ml medium
- Chemical Stability For one week at 40°C: 0.01 M HCl, 0.1 M NaOH For 12 h: 1 M NaOH, 70% acetic acid 30 min tested: 30% 2-propanol 1 h tested: 2% SDS
- Compatibility during use Stable in all commonly used buffers, reducing agents, denaturants, and detergents
- Supplied as suspension in 20% ethanol.
Storage and shipping: Shipped at ambient condition, refrigerate upon reciept.
Usage : bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemica