Description: : Purified, Staphylococcus aureus recombinant leukocidin-D (rLukD). The rLukD (tag free) is expressed in E. coli and purified by FPLC. The theoretical molecular weight of the protein is 36,889 Daltons.
Size: 100 ug of protein is supplied in PBS at a concentration of 1.085 mg/mL. Protein demonstrates a molecular weight of approximately 37 kDa. Endotoxin: 28.713 Endotoxin units/mg
Relevance: This protein may be used in functional toxicity assays in combination with rLukE, or as a control protein in ELISA assays or Western blotting when detecting toxins produced by different strains of S. aureus.
Recommended Dilutions:
ELISA: Assay-dependent dilution
WB: Assay-dependent dilution; internal QC demonstrates detection of 100 ng of rLukD protein using anti-PVL LukF polyclonal antibody (cat# 0312-002) in Western blotting.
Cytotoxicity assay: Cytotoxicity can be detected in human neutrophils when used in combination with LukE.
Storage: 2-3 weeks at -20°C, -80°C long term.
Intended for research use only, not for human, therapeutic, or diagnostic applications.