Description: : Purified, tag-free Staphylococcus aureus PantonValentine Leukocidin (PVL) S subunit (LukS-PV). The theoretical molecular weight of the protein is 32,465 Daltons
Size: 100 ug of protein is supplied in PBS at a concentration of 1.709 mg/mL. Protein demonstrates a molecular weight of approximately 35 kDa
Relevance: This protein may be used in functional PVL toxicity assays in combination with LukF-PV, or as a control protein in ELISA assays or Western blotting when detecting LukS in PVL (+) strains of S. aureus
Recommended Dilutions:
ELISA: Assay-dependent dilution
WB: Assay-dependent dilution; internal QC demonstrates detection of 100 ng of LukS-PV protein when detected with antiPVL LukS polyclonal antibody (cat# 04-0009) in Western blotting.
PVL Cytotoxicity assay: Cytotoxicity can be detected in human neutrophils when used in combination with LukF-PV in a concentration range of 1-100 nM.
Storage: 2-3 weeks at -20°C, -80°C long term.
Intended for research use only, not for human, therapeutic, or diagnostic applications.