What are autosampler vials used for?
Autosampler vials are used to hold samples for automated sampling in analytical instruments like HPLC (High-Performance Liquid Chromatography), GC (Gas Chromatography), and MS (Mass Spectrometry). They ensure accurate and consistent sample introduction into the instrument.
What materials are autosampler vials made of?
They are typically made of glass (clear or amber) or plastic (such as polypropylene), with glass being preferred for its inertness and compatibility with most solvents. Amber vials are used to protect light-sensitive samples.
How do I choose the right autosampler vial for my application?
When choosing the right autosampler vial for your applications, there are several factors to consider, including:
- Analysis – What type of analysis will you be using and is the vial suitable?
- Sample volume – Does the vial hold the amount of volume you are planning to use
- Light sensitivity – If using light sensitive samples, choose amber vials
- Vial material – Check the vial material is right for the application. If using samples that stick to glass, choose plastic or deactivated glass vials.
- Compatibility - Always check manufacturer specifications to ensure a good fit between the vial type and your autosampler model.
- Temperature – Consider the temperature you will be using and ensure the vial material can withstand the max temperature
- Seal – There are various seals available for autosampler vials, you should select the correct cap for your requirements
- Size – Ensure the size of the vial is suitable for the volume of the sample being used
What are the standard sizes of autosampler vials?
Common sizes include 1.5 mL (or 2 mL) vials, 4 mL, 10 mL, and 20 mL. The 1.5 mL size is the most widely used for routine analyses.
Why is vial sealing important?
Proper sealing prevents contamination, evaporation, and leakage, which can compromise sample integrity and lead to inaccurate results. Choose the correct cap and septum for your vial type to ensure a good seal.
What types of closures are available for autosampler vials?
Closures include screw caps, crimp caps, and snap caps. Screw caps are user-friendly and commonly used, while crimp caps provide a tighter seal but require special tools.
What are the common issues when using autosampler vials?
Common issues you may face when using autosampler vials include:
- Poor sealing & contamination
- Compatibility problems with autosamplers
- Static buildup
- Breakage
- Evaporation & sample loss
However, with proper selection, handling and correct storage, you can help to minimize experiencing these issues.
How should autosampler vials be stored?
It is important to store autosampler vials appropriately for safety and hygiene. Vials should be stored in a clean, dry place away from direct sunlight, especially for amber vials used with light-sensitive samples. You should also keep vials covered to avoid dust and contamination. Racks can be used, but ensure the rack fits the size of the vials you are storing.