Antigen: purified enzyme from mouse macrophages (RAW 264.7 cells)
Host: rabbit
Cross Reactivity: (+) iNOS from most mammalian species and nNOS (~5%); (−) eNOS
Application(s): ICC, IP, and WB
NOS catalyzes the biosynthesis of nitric oxide from L-arginine. iNOS is a soluble enzyme found in a variety of tissues including macrophages, hepatocytes, vascular smooth muscle cells, and chondrocytes. iNOS expression is increased by a variety of factors including LPS, IFN-g, IL-1b, and TNF-a.