Synonym(s): N-Succinimidyl (acetylthio)acetate; N-Succinimidyl S-acetylthioglycolate; S-Acetylthioglycolic acid NHS ester
Formula: C8H9NO5S
Formula Weight: 231.23
CAS No.: 76931-93-6
Purity: ≥95% (TLC)
Application: Thiolating reagent for primary amines; thiol can be deprotected under mild conditions. Typically, couples initially to a molecule containing primary amine by amide bond buffered at pH 7.5. Deprotection may be accomplished with 0.05 M hydroxylamine at neutral pH. Useful for preparation of enzyme immunoconjugates and hapten carrier molecule conjugates.
Other Notes: Note that the ester couples very efficiently to primary amines compared to other thiolating reagents. In addition, this reagent will neutralize original amine positive charge.
Storage: −20°C
Beilstein Registry Number: 7815491
Empirical Formula (Hill Notation): C4H10ClN · HCl