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Jackson Immunoresearch

Peroxidase AffiniPure Goat Anti-Rat IgM, µ Chain Specific

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Peroxidase AffiniPure Goat Anti-Rat IgM, µ Chain Specific

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the heavy chain of rat IgM but not with rat IgG or the light chains of rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, bovine and horse serum proteins, but it may cross-react with IgM from other species.

Target: Rat

Host: Goat

Antibody Format: Whole IgG

Specificity: IgM, µ Chain Specific

Minimal Cross Reactivity: Human, Bovine, Horse Serum Proteins

Conjugate: Horseradish Peroxidase

Clonality: Polyclonal

This product is for IN VITRO RESEARCH USE ONLY. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.

Physical StateFreeze-dried powder
StorageStore freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Note: after the addition of glycerol, the concentration of protein and buffer salts is one-half of the original. Alternatively, aliquot and freeze the product at -70°C or below in the absence of glycerol. Avoid repeated freezing and thawing. 
Expiration dateOne year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.
PurityThe antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. 
Buffer0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6  
Stabilizer15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free) 
PreservativeNone (Warning: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.) 
Suggested Working Concentration or Dilution Range
ELISA1:5,000-1:100,000
Western Blot1:5,000-1:100,000 (non-ECL) / 1:10,000-1:200,000 (ECL)
Histo-/Cyto-Chemistry1:500-1:5,000
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

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Product Detail
Thomas No.
C840P54
Mfr. No.
112-035-075
Description
Peroxidase AffiniPure Goat Anti-Rat IgM, µ Chain Specific (min X Hu, Bov, Hrs Sr Prot), 1mL
list price/quantitytotal
$0.00
$0.00 (0 Items)
Product C840P54 is restricted and can only be purchased by customers with a web profile linked to a Thomas Business Account - Click here to login or create your web profile. If you do not have a Thomas Business Account, please Contact Us and someone will respond with details on how to apply for a business account with us.

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