MDL No.: MFCD00162626
Storage: 2-8C
UNSPSC Code: 12352203
General description: IgG antibody plays a crucial role in humoral immune responses such as complement activation, phagocytosis, placental transport and cell surface-receptor binding. Anti-mouse IgG (Fc specific)-alkaline phosphatase antibody (diluted 1: 10,000 in PBS-Tween) can be used in ELISA. It is also useful in immunoblotting. Goat anti-mouse IgG (Fc specific)-alkaline phosphatase antibody reacts specifically with mouse IgG Fc fragment, IgG and its subclasses IgG1, IgG2a, IgG2b and IgG3 but not with human IgG, and mouse IgA or IgM.
Application: Alkaline phosphatase-conjugated goat anti-mouse Fc specific antibody was used as a secondary antibody in ELISA assays at a dilution of 1:1000 in PBS/0.1% Tween and 1% BSA for 1.5 hours at 37C. Antibody was developed using 4-nitrophenyl phosphate (Sigma) as a substrate for 30 minutes at 37C.
Immunogen: Purified mouse IgG Fc fragment.
Physical form: Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide
Preparation Note: Adsorbed to reduce background with human samples.
Other Notes: Antibody adsorbed with human IgG.
RIDADR: NONH for all modes of transport
WGK Germany: 2