Anti-Mouse IgG (Fc specific)-Alkaline Phosphatase antibody produced in goat, affinity isolated antibody, buffered aqueous solution

MDL No.: MFCD00162626

Storage: 2-8C

UNSPSC Code: 12352203

General description: Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice . Anti-Mouse IgG (Fc specific)-Alkaline Phosphatase antibody is specific for mouse IgG and mouse IgG, Fc fragment. The product does not react with mouse IgG, Fab fragment, and human IgG, IgA, IgM, κ and λ light chain. Additionally, the antibody does not bind to bovine IgG and IgM, or horse IgG.

General description: Mouse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in mouse serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.

Application: The product has been used as a secondary antibody for detecting mouse serum anti-hemagglutinin (HA) antibodies using ELISA techniques.

Application: Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.Enzyme-linked immunosorbent assay (1 paper)

Application: Alkaline phosphatase-conjugated goat anti-mouse Fc specific antibody was used as a secondary antibody in ELISA assays at a dilution of 1:1000 in PBS/0.1% Tween and 1% BSA for 1.5 hours at 37C. Antibody was developed using 4-nitrophenyl phosphate (Sigma) as a substrate for 30 minutes at 37C.

Application: Anti-Mouse IgG (Fc specific)-Alkaline Phosphatase antibody is suitable for use in ELISA (1:1500) and western blot. The antibody can also be used for immunohistochemistry (1:40 using formalin-fixed, paraffin-embedded sections).

Immunogen: Purified mouse IgG, Fc fragment

Physical form: Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide.

Preparation Note: Adsorbed to reduce background staining with bovine, horse, or human samples.

Other Notes: Antibody adsorbed with bovine, equine and human serum proteins.