Monoclonal Anti-Alkaline Phosphatase, Human Placental antibody produced in mouse, clone 8B6, ascites fluid

Synonyms: Monoclonal Anti-Alkaline Phosphatase, Human Placental; Anti-Phosphatase, Alkaline, Human Placental; PLAP

MDL No.: MFCD00162106

Storage: -20C

UNSPSC Code: 12352203

General description: Human placental alkaline phosphatase (EC 3.1.3.1; hPLAP) is an isoenzyme that is usually synthesized in the placental syncytiotrophoblast and becomes detectable in the maternal circulation after the twelfth week of pregnancy. Although hPLAP has been analyzed as a potential tumor marker, it has not been widely used in the routine clinical laboratory because of its low overall specificity due to heat-inactivation, cross-reactivity with intestinal AP and L-phenylalanine sensitivity. The use of monoclonal antibodies as reagents has greatly improved the sensitivity, reproducibility and accuracy of the tests used to distinguish placental isozymes from their tumor cell counterparts. Thus, monoclonal anti-alkaline phosphatase may be useful as diagnostic tools for tumor detection.

General description: In SDS gels, the product reacts with both Regan and Nagao isozymes of human placental alkaline phosphatase (hPLAP, 130 kDa, 67/130 kDa). By RIA, the antibody binds to hPLAP with an affinity constant of 5 x 109 LM-1. It does not react with PLAP-like enzymes.

Application: Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.Immunofluorescence (1 paper)Immunohistochemistry (1 paper)

Application: Cultured pancreatic islet cells were fixed with cold 4% paraformaldhyde at room temperature for 10 minutes and incubated with mouse monoclonal anti-Alkaline Phosphatase, Human Placental antibody at 1:500. Mouse monoclonal anti-Alkaline Phosphatase, Human Placental antibody was used to detect a unique molecular probe that consisted of the FGFR extracellular domain expressed as a chimera with human PLAP.

Application: Anti-Alkaline phosphatase human placental antibody coupled to agarose was packed in a column and used to purify alkaline-phosphatase conjugated FGF receptor out of conditioned media from COS-7 cells. Column was washed with 1M NaCl.

Immunogen: Human epidermoid carcinoma cell line expressing placental alkaline phosphatase.