Anti-SMAD2 pS467 affinity purified antibody has been tested for use in ELISA, DB, IHC, and IF. Expect a band approximately ~49, 52.3 kDa in size corresponding to human Smad2 protein by western blotting in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. Positive controls used: HeLa cells in Immunofluorescence and Human brain cortex in Immunohistochemistry.