2,2'-Azino-bis-(3-ethylbenzthiazoline-6-sulfonic Acid) Solution is oxidized to a radical action showing absorption maxima at 820 nm, 734 nm, 650 nm and 405 nm, in the presence of hydrogen peroxide and HRP. The latter frequency demonstrates a significant molar extinction coefficient and is generally employed for most ABTS assays. Stopping the reaction with acid does not alter the 405 nm spectrum allowing kinetic and endpoint methods to be measured at the same wavelength. Provided as a room temperature stable, single component ABTS solution satisfactory for quantitative analysis of HRP based systems.
2,2'-Azino-bis-(3-ethylbenzthiazoline-6-sulfonic Acid) solution is considered as a safe-sensitive stabilized liquid substrate for horseradish peroxidase (HRP) based ELISA assays. It may be used for both kinetic and endpoint reactions.
It must be warmed as assay temperature prior to use. Discard if solution becomes bright yellow or turbid. Protect from exposure to direct sunlight. 0.625 mmol/L Oxalic Acid solution is recommended as a stopping solution.
Key Applications: Stabilized Liquid Substrate for ELISA
Application Areas: Immunoassays
Product Type: Reagents
Biochemical Category: Reagents
Presentation: Solution
Format: Solution
Formulation: Contains ABTS, 1.46 mMol L -1, in a citrate buffer, pH 4.0. Also contains stabilizer
pH: 4.0 ± 0.05 (Lit.)
Storage & Handling: +4°C