IsoPol™ DNA Polymerase is active at ambient temperatures.
It exhibits 5’-3’ polymerase activity and lacks 3’-5’ exonuclease activity. The 5’-3’ exonuclease domain is truncated, thus the enzyme also lacks 5’-3’ exonuclease activity. IsoPol™ DNA Polymerase has excellent processivity and strong stand-displacement activity.
In addition, it can be heat inactivated at temperatures above 50°C.
Properties
Unit Definition: One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C. IsoPol™ DNA Polymerase exhibits a specific activity of ~15000 U/mg.
Relative specific activity: The relative specific activity of the IsoPol™ DNA Polymerase was compared to other commercially available polymerases by using assay conditions as mentioned in the unit definition above.
Strand-displacement activity: IsoPol™ DNA Polymerase exhibits similar strand-displacement activity as that of Bsu DNA polymerase I at 25°C.
Optimal reaction conditions: Optimal performance is achieved in a pH 8.5 buffer supplemented with 5 mM MgCl2 and 25-100 mM salt (NaCl or KCl), at 37°C.
Storage and Stability: The enzyme is stable at -20°C for > 1 year in the supplied storage buffer. The enzyme tolerates at least five freeze-thaw cycles (-80°C) without loss of activity.
Quality Control
ArcticZymes is dedicated to the quality of our products. We manufacture all our products at our ISO 13485 certified facility in Norway
dsDNA endonuclease activity
25 U IsoPol™ DNA Polymerase was incubated with a supercoiled plasmid (1 µg) for 4 hours at 37°C. Agarose gel electrophoresis did not reveal any transformation of closed circular DNA to nicked DNA.
ssDNA endonuclease activity
25 U IsoPol™ DNA Polymerase was incubated with M13 ssDNA (0.5 µg) for 4 hours at 37°C. Agarose gel electrophoresis did not reveal any visible signs of ssDNA degradation.