IsoPol® SD+ is a DNA polymerase with stronger strand displacement and higher salt tolerance, compared to ArcticZymes first IsoPol™.
Properties
Unit Definition: One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C. IsoPol® SD+ exhibits a specific activity of ~7 500 U/mg.
Strand-displacement activity: IsoPol® SD+ exhibits stronger strand-displacement activity than other polymerases at 25°C and 37°C.
Features: IsoPol® SD+ exhibits 5’-3’ polymerase activity and lacks 3’-5’ exonuclease activity. The 5’-3’ exonuclease domain is truncated, thus the enzyme also lacks 5’-3’ exonuclease activity.
Inactivation: IsoPol® SD+ can be heat inactivated at temperatures above 50°C.
Optimal reaction conditions: Optimal performance is achieved in a pH 8.5 buffer supplemented with 4-6 mM MgCl2 and 100-350 mM salt (NaCl or KCl), at 37°C.
Storage and Stability: The enzyme is stable at -20°C for 3 years in the supplied storage buffer. The enzyme tolerates at least four freeze-thaw cycles (-80°C) without loss of activity. Storage of the enzyme at 200 mM NaCl is recommended for high concentration samples.