SAN High Quality 2.0 is the second generation of our SAN High Quality enzyme. SAN HQ 2.0 offers wider compatibility with downstream processes used in bioprocessing workflows while exhibiting the same salt tolerance and other biochemical characteristics as SAN HQ.
The downstream compatibility improvements are of particular importance if used with anionic exchange media or using size exclusion chromatography / TFF below 100 kDa pore size.
Properties
- Source: Recombinantly produced in Pichia pastoris
- Molecular weight: 26 kDa
- Protein purity: > 99% by SDS-PAGE analysis
- Isoelectric point: 9.55
- Unit definition: One unit is defined as the amount of enzyme that causes a ΔA260 = 1.0 in 30 minutes at 37°C in 25 mM Tris-HCI pH 8.5 (@25°C), 5 mM MgCl2, 500 mM NaCl, and 50 μg/ml calf thymus DNA.
- Specificity: Nonspecific endonuclease cleaving single- and double stranded DNA and RNA. The enzyme degrades DNA vs RNA in a 10:1 ratio.
- Working ranges:
- Temperature: 7 – 38°C, 4°C overnight, optimal: 30 – 38°C
- Salt concentration (NaCl / KCl): 100 - 900 mM, optimal: 400 - 650 mM
- Mg2+: >1 mM is required for activity, optimal 5 - 50 mM
- pH: 7.3 – 9.2, optimal 8.2 - 8.8
Note: The working range is defined as ~10% of activity and optimal range is ~80% of activity.
- Tolerance to typical buffer additive:
Triton X-100: No reduction in activity (tested up to 15%)