Stain for detection and location of proteins.
Protocol: Wash blot in 0.#% Tween/PBS, with three changes of 15 min. each at RT. Place blot in stain solution for 5 min. at RT with agitation. Destain in 90% methanol/2% acetic acid/8% water.
Stain for proteins and total carbohydrates in plants.
A histological, protein, and nucleic acid dye used in electrophoresis
High Purity Grade EM (615nm, H2O) > 28,500
Synonym: Acid Black 1; Amido Black 10B; Buffalo Black NBR
Refractive index: n20D 1.77 (Predicted)
Appearance: Dark red to black powder
Density: 1.05 g/mL at 20°C
HS Code: 3204.12
IUPAC_Name: disodium (6Z)-4-amino-3-(4-nitrophenyl)diazenyl-5-oxo-6-(phenylhydrazinylidene)naphthalene-2,7-disulfonate
Melting Point: > 350°C
RTECS Number: QJ6196000