SPINeasy RNA Kit for Bacteria is a silica -membrane spin -column kit that enables quick and convenient purification of total RNA from microbial samples, including bacteria and fungi. Included in the kit is our specially formulated RNASS solution that stabilizes and protects RNA. The use of Lysis Buffer R and Lysing Matrix B in combination with FastPrep instruments from MP Biomedicals enables highly efficient lysis of microbial samples within seconds. With a simple workflow, the kit allows multiple samples to be processed simultaneously, without the use of toxic substances such as phenol and chloroform. Total RNA of high quality and integrity can be purified typically within 40 minutes and is immediately available for RT-PCR and other downstream applications.
Application Notes:
The SPINeasy RNA Kit for Bacteria quickly and efficiently isolates high-quality, total RNA from G+ and G- bacteria using a specialized Lysing Matrix B for cell lysis and SPIN columns for the purification process.
Usage Statement:
Research Use Only (RUO)
Key Applications:
Total RNA extracted from SPINeasy RNA Kit for Bacteria can be used in several downstream applications, including RT-PCR, qPCR, Sequencing (NGS), Microbial transcriptomics, RNA-Seq, Northern Blot Analysis, Genomics, Molecular Biology
Key features and details
- Quick and convenient purification of total RNA from microbial samples using spin column technology
- Lysing Matrix B and specially formulated Lysis Buffer for efficient and reproducible sample lysis with FastPrep instruments
- Highly purified RNA, suitable for RT-PCR, qPCR, Sequencing (NGS), and Northern Blot Analysis
- Safe - no phenol/chloroform extractions
Alternate Names: RNA Extraction Kit for Microbes; Total RNA Extraction Kit for Bacteria and Fungi
Application Notes: The SPINeasy RNA Kit for Bacteria quickly and efficiently isolates high-quality, total RNA from G+ and G- bacteria using a specialized Lysing Matrix B for cell lysis and SPIN columns for the purification process.
Base Catalog Number: 65410
Brand: SPINeasy
Components: 50x Lysing Matrix B, 15 mL RNASS, 60 mL Lysis Buffer R, 6 mL Wash Buffer R, 10 mL Nuclease-free water, 1 vial DNase I, 5 mL DNase I Buffer, 50x Column R with collection tube
Hazard Statements: Hazard Class 8
Pack Size: 50 Preps
Personal Protective Equipment: Safety Goggles/Faceshield; Gloves
Sample Type: Bacteria (Gram +/-), Fungi, Yeast
Storage: Store at Room Temperature (15-25 °C) in a dry environment
Usage Statement: Research Use Only (RUO).