Features & Benefits:
Unbiased Lysis: Efficient and unbiased lysis of microbes including gram positive/negative bacteria, fungi, protozoans, and viruses from any sample including feces, soil, plant, water, biofilms, swabs, saliva, body fluids, etc.
Ultra-Pure: High-quality total RNA (including small/microRNAs) is inhibitor-free and ready for RT-PCR and microbiome measurements using Next-Gen Sequencing.
High-Sensitivity: Increased detection limit of very low abundance organisms.
The ZymoBIOMICS MagBead RNA kit provides a high-throughput, magnetic beadbased purification of total RNA (including small/microRNAs) from any sample source (e.g., microbes including gram positive/negative bacteria, fungi, protozoans, viruses, fecal, soil, plant, water, biofilms, swabs, saliva, body fluids and etc.). The provided DNA/RNA Shield inactivates infectious agents and is ideal for sample storage at ambient temperatures. Total RNA is eluted into ≥50 µl of ZymoBIOMICS DNase/RNase-Free Water and is ready for any downstream application including Next-Gen Sequencing, RT-qPCR, etc.
Binding Capacity 15 µg total RNA per 30 µl MagBinding Beads.
Equipment Needed Magnetic stand, heat block, liquid handler or robotic sample processor (user provided).
Purity High-quality RNA is ready for Next-Gen Sequencing, RT/PCR, hybridization, etc.
Recommended Materials (sold separately) – 96-well Collection Plate (C2002; capacity is up to 1.2 ml/well), 96-Well Block (P1001; capacity is up to 2 ml/well), 96-well Elution Plate (C2003), Cover Foil (C2007), ZR-96 MagStand (P1005).
Sample Preservation DNA/RNA Shield lyses cells, inactivates nucleases and infectious agents and is ideal for safe sample storage and transport at ambient temperatures.
Sample Types Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA and RNA are efficiently isolated from ≤ 50mg of mammalian feces, ≤ 50mg soil, and 5-20mg (wet weight) of fungal bacterial cells, biofilms, water, and swabs.
Size Limits Total RNA including small/microRNAs ≥ 17nt.
Storage RNA eluted with DNase/RNase-Free Water (provided) can be stored frozen. The addition of RNase inhibitors is highly recommended for prolonged storage.