RT-PCR-based detection kits for Hop Latent Viroid (HLVd)
Can be used with purified RNA from a variety of sample types
Available in real-time TaqMan format
Real-time format includes internal control and synthetic positive control to ensure your assay is working properly every step of the way
Pair with Norgen's Plant/Fungi RNA Purification Kit for the highest level of sensitivity in detection
Detect HLVd present in plant crops - Discover Now
Hop latent viroid (HLVd) is a single-stranded, circular infectious RNA of 256 nucleotides. Initially it was believed that this viroid did not produce any symptoms of disease, thus the inclusion of the term latent in the viroid name. However it is now known that HLVd infection occurs worldwide in hop (Humulus lupulus) and is deleterious to production in certain cultivars. Quantitative changes in some secondary metabolites in the secretory glands forming lupulin are induced by HLVd infection, indicating that the viroid is not truly latent. Hop Latent Viroid infection depends on the metabolism of the host plant, and it’s replication and pathogenesis are mediated by its structural features and its ability to interact with cellular factors.
HLVd TaqMan RT-PCR Kit, 100 reactions
Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
Specific Primer and Probe mix for the pathogen/virus/viroid of interest
Primer and Probe mix
Positive and negative control to confirm the integrity of the kit reagents
HLVd TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions
Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
Nuclease-free water
Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix
For research use only and NOT intended for in vitro diagnostics.