Phosphotyrosine is a tyrosine residue covalently bound to a phosphate via its hydroxyl group. A tyrosine kinase catalyzes the transfer of a phosphate from ATP to a tyrosine residue on the protein substrate. Tyrosine phosphorylation plays a key role in intracellular signaling and cancer development. Many tyrosine kinases are drug targets for different forms of cancer. Phosphotyrosine antibody specifically binds to phosphotyrosine residues. It is a valuablel tool to analyze tyrosine phosphorylation and monitor the activity of tyrosine kinase in high throughput drug discovery. THE™ Phosphotyrosine Antibody (E10) plus recognizes a broadspectrum of phosphotyrosine-containing proteins from numerous species.
GenScript THE™ Phosphotyrosine Antibody (E10) plus is an anti-Phosphotyrosine monoclonal antibody (5E10E1) mixed with another anti-Phosphotyrosine monoclonal antibody (18E10D2) at an optimal ratio. The antibodies are produced from a hybridoma resulting from the fusion of SP2/0-Ag14 myeloma and B-lymphocytes harvested from mouse immunized with phosphotyrosine conjugated to KLH.