The LavaLAMP™ DNA Master Mix is intended to simplify development and optimization of DNA LAMP (loop-mediated isothermal amplification) reactions. LAMP kits are commonly available as multi-component kits that require optimization (e.g. MgSO4, betaine, enzyme as well as temperature, primer concentration, etc.). The LavaLAMP™ DNA Master Mix greatly simplifies reaction optimization by limiting optimization to target specific components/conditions such as LAMP primer design, target concentration and reaction temperature. Additionally, this master mix is heat stable at 90°C for =5 minutes, which enables the addition of a reaction preheating step which may, depending on the target, increase assay sensitivity and decrease time to results.