Pfu DNA Polymerase, 5u/ul: Pfu DNA Polymerase, 5u/ul
Product Description:
Cloned Pfu DNA polymerase is isolated from the hyperthermophilic marine archaebacterium. Pyrococcus furiosus. The multinational thermostable enzyme possesses both 5'- to 3'- DNA polymerase and 3'- to 5'- exonuclease activity, which results in a 12-fold increase in fidelity of DNA synthesis over Pfu DNA polymerase.
Cloned Pfu DNA Polymerase has a temperature optimum between 72°C and 78°C remains > 95% active following one hour incubation at 95°C.
10 x Reaction Buffer:
200 mM TrisHCl ( pH 8.8)
100 mM KCl
100 mM (NH4)2 SO4
20 mM MgSO4
1% Triton X-100
1 mg / ml nuclease-free bovine serum albumin (BSA)
Unit Activity:
One unit of activity is the amount of cloned Pfu DNA polymerase required to incorporate 10nM of [H] TTP into an acid-insoluble form in 30 minutes at 72°C.
Contaminants:
Cloned Pfu DNA polymerase is tested extensively for the absence of detectable nonspecific nuclease activity and DNA contamination.