RNA 5´ Polyphosphatase is a Mg2+-independent phosphohydrolase discovered and characterized by Epicentre scientists. The enzyme sequentially removes the ? and ß phosphates from 5´-triphosphorylated RNA (such as primary RNA transcripts): 5´ pppN—OH 3´ ′→ 5´ pN—OH 3´ + 2 Pi