Synonyms: 2′-Deoxyguanosine 5′-triphosphate trisodium salt solution; dGTP-Na3
Empirical Formula (Hill Notation): C10H13N5Na3O13P3
Formula Weight: 573.13
Linear Formula: C10H13N5Na3O13P3
MDL Number: MFCD00080337
Purity: <0.9% (dNDP); 99% (dNTP)
Storage: −20°C
UNSPSC Code: 12352200
RIDADR: NONH for all modes of transport
Application:
2′-Deoxyguanosine 5′-triphosphate trisodium salt is utilized for methods such as DNA sequencing, PCR and other DNA polymerase based DNA synthesis and repair techniques.
Biochem/physiol Actions:
2′-Deoxyguanosine 5′-triphosphate is used in vivo synthesis for the synthesis of DNA via DNA polymerases.
General description:
dGTP, PCR Grade, is a 100 mM clear, colorless solution of the sodium salt (pH 8.3). PCR Grade nucleotides from Roche are specially manufactured and purified to the highest possible chemical purity. PCR Grade nucleotides contain at least 99% of the relevant deoxynucleoside-triphosphate (dNTP) and less than 0.9% deoxynucleoside-diphosphate (dNDP).
Other Notes:
For general laboratory use.
Quality:
Function tested in RT-PCR. Tested for absence of contaminating deoxyribonucleases, ribonucleases and nicking activities according to the current Quality Control procedures.
Physical form:
solution of dGTP in water (pH 8.3).
Preparation Note:
Working solution: We recommend to prepare a deoxynucleotide mix containing dATP, dCTP, dGTP, dTTP (10 mM, each); (e.g., for the preparation of 100 μl nucleotide mix add 10 μl of dATP, dCTP, dGTP, dTTP (each) to 60 μl Water, PCR Grade).