Animal-free Recombinant Human IL-7 is expressed in Human 293 cells as a highly glycosylated protein. Under reducing conditions, IL-7 migrates as three separate bands as 19 kDa, 25 kDa and 30 kDa. In comparison IL-7 produced in an E. coli expression system have glycosylation and migrate as a single band at 17 kDa. IL-7 is a hematopoietic cytokine that is important for B and T cell development. When combined with other factors, IL-7 can also co-stimulate the proliferation of mature T cells. Production in human 293 cells uses a serum-free, chemically defined media and offers authentic glycosylation.