This kit includes pooled virus representing each of the 2 possible capture sequences and 2 position combinations (capture sequences 1 or 2, in the stem-loop or 3 position). Present within each pool are 2 CRISPRi sgRNAs, 1 targeting RAB1A and 1 non-targeting control sgRNA. Each pool is provided ready to use at 1x106 viral titer, sufficient enough to cover 2x106 cells at an MOI of 0.5. The purpose of the optimization kit is to set up a pilot screen to determine the best capture sequence and position configuration before a custom pool is ordered.We have an exclusive partnership with 10x Genomics & our compatible CRISPRi pools are a perfect complementary product for target gene validation following a whole genome screen.The 10X CRISPRi Feature Barcode Optimization kit is ideally suited for setting up the screen assay and if desired capture sequence optimization before using the Custom 10x Compatible CRISPRi library pools. For more information about single cell screening with CRISPR and 10x genomics Click Here.
Storage: -70C
Application:
•Optimization kit for 10x CRISPRi screening
•Pooled CRISPR Screening with 10x Genomics Compatibility
•Strong transcriptional inhibition in multiple cell lines
•Creation of cell lines stably expressing KRAB-dCas9
•Validated positive and negative controls
Legal Information: CRISPR Use License AgreementLentiviral, WPRE and Evrogen Fluorophore Licenses