- Generates A-overhangs
- Ideal for long PCR, up to 10 kb targets
- 10X greater fidelity than native Taq
- Ideal for difficult, high GC content sequences
Accuris High Fidelity Hot Start Master Mix is a hot start 2X formulation which provides excellent sensitivity in low copy number assays with 10X higher fidelity than Taq polymerase. The 2X master-mix contains the same enhancers as the Accuris High Fidelity Master Mix but with an added hot start mechanism.
The mix is supplied in a single tube, reducing the number of pipetting steps while improving throughput and reproducibility. The highly efficient buffer formulation and hot-start enzyme blend provides the ideal conditions for high-performance PCR. The mix is inactive at room temperature, thereby eliminating non-specific amplification.