Synonyms: Anti-GAD 65
MDL No.: MFCD03455043
Storage: -20C
UNSPSC Code: 12352203
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General description: GAD is predominantly expressed in the central nervous system (CNS) and pancreatic islets. It is also expressed in testis, oviduct and ovary. GAD exists in two isofoms, GAD 65 and GAD 67. GAD65 is targeted to membranes and nerve endings.
Application: Anti-Glutamic Acid Decarboxylase 65 antibody produced in rabbit is suitable for use as a primary antibody at a working dilution of 1:1000 in fluorescent immunohistochemistry of stomach and jejunum sections of mice. It is suitable for immunoblotting at a working dilution of 1: 4000 using a rat brain extract. It is also suitable for indirect peroxidase immunohistochemical staining of formalin-fixed, paraffin-embedded sections of rat pancreatic islet cells at a working dilution of 1:1000.
Biochem/physiol Actions: Glutamic Acid Decarboxylase (GAD) catalyzes the conversion of L-glutamate to γ-aminobutyric acid (GABA), the principal inhibitory neurotransmitter in the brain, and a putative paracrine signal molecule in pancreatic islets. GAD65 is an ampiphilic, membrane-anchored protein, (585 amino acid residues) and is encoded on human chromosome 10. It has been identified as an autoantigen in insulin-dependent diabetes mellitus (IDDM) and stiff-man syndrome (SMS) and may serve as a marker in the early stages of IDDM. GAD 65 synthesizes transmitter GABA for vesicular release.
Immunogen: synthetic peptide corresponding to the N-terminal region of human GAD 65 (amino acids 5-22). The sequence is highly conserved in rat and pig GAD 65 (single amino acid substitution) and mouse and monkey GAD 65, but is not found in GAD 67.
Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Specificity: Reacts specifically with GAD 65 (65 kDa).