MDL No.: MFCD03455078
Storage: -20C
UNSPSC Code: 12352203
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General description: Guanylyl cyclase (GC) catalyzes the conversion of guanosine-5'-triphosphate (GTP) to cyclic guanosine-3',5-monophosphate (cGMP) and pyrophosphate. This reaction requires Mg2+ or Mn2+. The enzyme consists of α and β subunits of ~80kDa and ~70kDa respectively. Both the units are required for catalytic activity. The N-terminal domains of the subunits are essential for the stimulation of the enzyme by NO. Dimerization is mediated by the central portion of GC. The C-terminus domain of both subunits forms the catalytic domain.
Application: Anti-Guanylyl Cyclase β1 (ER-19) antibody produced in rabbit is suitable for immunoblotting at a working dilution of 1:2000 using a cytosolic fraction of rat brain, immunoprecipitation at a working antibody amount of 2-3μg using 60-120μg of a cytosolic fraction of rat brain and for immunohistochemistry at 1:400 using trypsin-digested human, bovine and mouse heart tissue. It was used as a primary antibody for immunohistochemical . localization of β1 subunits of sGC (soluble guanylate cyclase) in the guinea pig gastrointestinal tract. detection of expression of sGC in the vasculature of rat skeletal muscle. localization of the functional subunit of NO receptors, sGCβ1 in guinea pig caecumIt was used in immunoblot assay to determine whether there were changes in uterine vascular smooth muscle (UVSM) sGC expression during the ovine reproductive cycle.
Biochem/physiol Actions: The enzyme (GC) is a major physiological receptor for nitric oxide (NO), an important intra- and intercellular membrane-permeant signaling molecule. Gaseous NO binds to Fe2+ in the prosthetic heme group of the enzyme. NO binding is followed by disruption of the β1 subunit histidine105 bond to iron and activation of the enzyme. GC forms a complex with NO and cGMP and regulates smooth muscle relaxation, inflammation, platelet adhesion and aggregation, pulmonary physiology and neuronal function. It is an important target for NO-releasing and non-NO-releasing activator drugs in human cardiovascular therapy.
Immunogen: synthetic peptide corresponding to amino acid residues 189-207, with N-terminal added lysine, of rat soluble guanylate cyclase 1, conjugated to KLH with glutaraldehyde. The sequence differs in human, bovine and mouse by 1 amino acid.
Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
RIDADR: NONH for all modes of transport
WGK Germany: 3