The BlitzAmp Advanced Hotstart 1-Step Probe Master Mix Kit is a reagent system for one-step RT-qPCR analysis using any gene-specific primer and probe set(s) in single-plex or multiplex assay design. It is optimized for highly sensitive and robust amplification and detection over a wide range of RNA template inputs with a rapid turnaround time. With the higher concentration 4X master mix, users have the flexibility to input more sample per reaction for the desired level of sensitivity even in low-volume reactions.