SIGMA BIS-TRIS propane PharmaGrade, Manufactured under appropriate controls for use as a raw material in pharma or biopharmaceutical production, suitable for cell culture

PharmaGrade products are manufactured under appropriate controls for use as a raw material in pharma or biopharmaceutical production. For more information on the PharmaGrade Program, visit sigma-aldrich.com/safc-pharmagrade.

Synonyms: 1,3-Bis[tris(hydroxymethyl)methylamino]propane

Molecular Formula: C11H26N2O6

Molecular Weight: 282.33

Linear Structural Formula: CH2[CH2NHC(CH2OH)3]2

MDL Number: MFCD00004689

Melting Point: 164-165 C (lit.)

Application: Bis Tris Propane is a unique biological buffer also referred to as a "Universal" buffer, which means the buffer capacity covers a wide range. Bis Tris Propane has two pKas of 6.3 and 9.5, which expands the buffering range from 5.0-10.5-making this a versatile buffer for biological systems. Bis Tris Propane is considered to be non-toxic to culture cell lines and provides high-solution clarity.A review of DNA polymerase techniques lists bis tris propane as a suitable buffer for PCR methods as a universal buffer pH gradient is employed.