Synonyms: 7-Deaza-2′-deoxyguanosine 5′-triphosphate lithium salt; −N7-dGTP; 7-Deaza-dGTP
Empirical Formula (Hill Notation): C11H17N4O13P3
Formula Weight: 506.19
Linear Formula: C11H17N4O13P3
MDL Number: MFCD00171614
Purity: ≤4% (7-Deaza-dGDP, HPLC); 95% (7-Deaza-dGTP, HPLC)
Storage: −20°C
UNSPSC Code: 41116100
RIDADR: NONH for all modes of transport
Analysis Note:
Absorption: 25 A260 units of product correspond to 1 mg.
Application:
7-Deaza-dGTP is a substrate for most DNA polymerases, including Taq DNA polymerase.
7-Deaza-dGTP is used in the dideoxy-chain termination sequencing methods, in place of dGTP to overcome compression problems in gel electrophoresis when sequencing GC-rich stretches of DNA.
Partial substitution of 7-Deaza-dGTP for dGTP in PCRs can improve the product yield when the template is GC-rich (i.e., has extensive secondary structures).
General description:
7-deaza-2′-deoxy-guanosine-5′-triphosphate is a nucleotide analog that is a candidate substrate for processive telomerase. The use of this analog improves PCR (polymerase chain reaction) yields. This compound also makes sequencing of GC rich sequences easier, especially if template amount is low and/or DNA quality is not good.
Other Notes:
When PCR products contain 7-Deaza dGTP, they do not stain well in ethidium bromide, presumably because stacking of adjacent bases in the products is diminished.
PCR amplification efficiency is generally not affected by 7-Deaza-dGTP.
For life science research only. Not for use in diagnostic procedures.
Preparation Note:
Working solution: Dilute 7-deaza-dGTP into a solution containing 50 mM Tris, pH 7.0, and use at exactly the same concentration as you would use dGTP.