Deoxyribonuclease I bovine, recombinant, expressed in Pichia pastoris, buffered aqueous glycerol solution, >=5,000 units/mg protein

Synonyms: DNAse I; Deoxyribonucleate 5'-oligonucleotido-hydrolase

MDL No.: MFCD00130918

Storage: -20C

Enzyme Commission (EC) Number: 3.1.21.1  ( BRENDA | IUBMB )

UNSPSC Code: 12352204

Features and Benefits: . RNA purification by removing DNA. Prepare DNA for nick translation1. Footprinting assays to determine DNA-protein interactions2

Application: Deoxyribonuclease I bovine has been used in a study to compare the initial actions of spleen deoxyribonuclease and pancreatic deoxyribonuclease. Deoxyribonuclease I bovine has also been used in a study to investigate deoxythymidine 3', 5'-di-p-nitrophenyl phosphate as a synthetic substrate for bovine pancreatic deoxyribonuclease.

Application: DNAse I from Sigma was used to treat nuclear lysate to obtain single nucleosomes in a study. The enzyme has also been for the preparation and harvest of mice mammary glands.

Application: Used for the removal of DNA from protein samples.

Biochem/physiol Actions: DNase I is an endonuclease that acts on phosphodiester bonds (adjacent to pyrimidines) to produce polynucleotides with terminal 5′-phosphates. The pH optimum is found to be between 7 and 8. Divalent cations such as Mn2+, Ca2+, Co2+, and Zn2+ are activators of the enzyme. A concentration of 5 mM Ca2+ stabilizes the enzyme against proteolytic digestion. 2-Mercaptoethanol, chelators, sodium dodecyl sulfate (SDS) and actin are known to inhibit the enzyme activity.

Biochem/physiol Actions: Digests single- and double-stranded DNA to a mixture of mono- and oligonucleotides carrying 5' phosphates and 3' OH termini. This catalytic activity is divalent ion-dependent. In the presence of Mg2+, DNase I hydrolyzes each strand of double-stranded DNA randomly and independently. In the presence of Mn2+, both strands can be cleaved.

Physical form: Supplied as a solution in 4 mg/ml glycine pH 5.0, 5 mM calcium acetate and 50% glycerol

Unit Definition: One unit will produce a DeltaA260 of 0.001 per min per mL reaction mixture using calf thymus DNA at pH 5.0 and 25C

Preparation Note: Produced without using any animal cells or animal derived materials.