Lipids are structural components of cell membranes that play a critical role in gene transcription, signaling, and metabolism. Several lipid species exist in biological systems, including phospholipids, triglycerides, and fatty acids. When extracting fatty acids at normal or high levels, such as in food matrices, use the MAK338 fatty acid extraction kit. This kit produces stronger signals. If in doubt about which kit to use, use MAK338. When extracting fatty acids at low or very low levels, such as in cellular matrices, use the MAK174 fatty acid extraction kit. The Folch method has been conventionally used to extract lipids containing fatty acids from biological samples, using chloroform, methanol, and water to separate lipids from aqueous-soluble compounds. In this procedure, lipids are retained in the lower chloroform layer; whereas, aqueous-soluble compounds are retained in the upper methanol-water layer. The sample is then centrifuged to achieve uniform separation and the bottom chloroform layer is transferred with a pipette to a new test tube.The Fatty Acid Extraction Kit shortens this extraction process by eliminating the need to prepare solvents and standards, centrifugation, and pipetting. Once the sample is homogenized and dissolved in the Extraction Solvent supplied in our kit, it is inverted twice and poured into the syringe containing a filter, which preferentially elutes the chloroform layer containing total lipids. A portion of the total lipid extract containing fatty acids can then be transesterified for GC-FID analysis.We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product reduces solvent waste over traditional methods. Find details here.
Storage: room temp
Other Notes: Experts have tested the Fatty Acid Extraction Kit. Watch the Video to learn more and to see how the technology works.