Synonyms: APO-1 interacellular domain; CD95 intracellular domain; Fas IC
MDL No.: MFCD00676999
Purity: >90% (SDS-PAGE)
Storage: -70C
UNSPSC Code: 12352200
General description: Fas is a key component of apoptosis, and is a cell surface receptor. This gene is localized to human chromosome 10q24.1, and is composed of nine exons and eight introns.
Biochem/physiol Actions: Fas and its ligand play an essential role in apoptosis, by activating the cell death signaling pathway. Mutations in this gene are associated with evasion of apoptosis by cells, leading to tumorigenesis, and prevention of clearance of these cells by the immune system. A polymorphism in this gene results in the production of soluble Fas (sFas), which is implicated in the chronic autoimmune disorder called primary Sjögren′s syndrome (pSS).
Biochem/physiol Actions: Fas intracellular domain, amino acids 191-335, is a widely expressed membrane receptor, which is responsible for controlling tissue homeostasis and immune responses. Fas receptor is activated by Fas ligand causing its trimerization. The aggregated Fas intracellular death domain recruits, via FADD adapter, pro-caspase-8 and forms a DISC (death-inducing signaling complex). Caspase 8, in turn, activates the downstream caspases, such as caspase 3, culminating in apoptosis.
Physical form: Solution in 50 mM Tris-HCl, pH 8.0, 2 mM EDTA, 2 mM DTT, 300Â mM NaCl, 0.05% NP-40, and 10% sucrose.