Fpg Protein from Escherichia coli, >=90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution, >20,000 units/mg protein

Synonyms: Fapy DNA glycosylase; Formamidopyrimidine DNA glycosylase; MutM

CAS Number: 78783-53-6

MDL No.: MFCD02264758

Purity: >=90% (SDS-PAGE)

Storage: -20C

Enzyme Commission (EC) Number: 3.2.2.23  ( BRENDA | IUBMB )

UNSPSC Code: 12352202

General description: Formamidopyrimidine-DNA glycosylase (Fpg) is a DNA repair enzyme found in Escherichia coli, which contains one zinc atom. Proximal to its C-terminal, it contains a zinc-finger motif of CC/CC type.

Application: Fpg Protein from Escherichia coli has been used for the assessment of DNA oxidative damage using comet assay.

Biochem/physiol Actions: Formamidopyrimidine-DNA glycosylase (Fpg) cleaves double-stranded DNA containing the damaged base 8-oxo-7,8-dihydroguanine. It functions as an N-glycosylase and apurinic/apyrimidinic lyase.

Physical form: Solution in 50% glycerol containing 50 mM potassium HEPES, pH 7.5, 1 mM DTT, 1 mM EDTA, and 200 mM NaCl.

Unit Definition: One unit will cleave 50% of 0.5 pmol of double-stranded DNA oligomer substrate (8-oxoguanine-mutated) in 10 min at 25 C.