Product Source: Human donor plasma.
Purity: ~95% (SDS-PAGE)
Storage: 2-8C
Application: Immunochemical standard Cell attachment and proliferation assays on human endothelial cells, human keratinocytes and human dermal fibroblasts Suggested Procedure for Coating Cell Cultureware 1.Determine the amount of HFN needed to coat culture vessels by multiplying the total surface area (cm2) by the desired concentration (mug/mL) of HFN. Recommended amount is 2-10 mug/cm2. 2.Wet the surface of each culture vessel to be coated with a minimum amount of sterile balanced salt solution (serum and protein free) required to cover the entire area. 3.Introduce the proper CO2 atmosphere, if required. 4.Add the calculated amount of HFN to each culture vessel. 5.Allow HFN to adsorb to the surface of the vessel for 5-20 minutes. 6.Remove residual balanced salt solution before proceeding with standard cell culture procedures
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