Intact Genomics igMax™ DH10B derivative ElectroCompetent cells are suitable for demanding cloning situations, such as synthetic bio-applications, BAC cloning, assembling large and multi-DNA fragments, and large construct transformation or cloning difficult targets, requiring the greatest number of transformants possible. Utilizing proprietary manufacturing methods, these cells allow for effective transformation of all large DNA molecules (≥10kb up to 350kb!).
Product Includes:
- igMax™ DH10B electrocompetent cells
- pUC19 control DNA
- Recovery medium
Genomic Features:
• Φ80lacZΔM15 marker provides α-complementation of the β-galactosidase gene with blue/white screening
• mcrA genotypic marker and the mcrBC, mrr deletion allows for cloning DNA that contains methylcytosine and methyladenine
Genotype:
F – mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 φ80dlacZ?M15 ΔlacX74 araD139 Δ(ara, leu)7697 galU galK rpsL (StrR) nupG λ
Product Includes:
• DH10B Electrocompetent cells
• pUC19 control DNA
• Recovery medium