In addition to studying inflammation for dog nutrition and disease research, canine animal models are important for human biomedical research and drug development. Cytokine and chemokines are molecular mediators of immune cell response in inflammation and immunity. Quantification of cytokines and chemokines is essential to understanding the immune system, as well as for researching disease states such as autoimmune, allergic reactions, sepsis, and cancer. The MILLIPLEX® Canine Cytokine Panel is 13-plex kit to be used for the simultaneous quantification of any or all of the following analytes in canine serum, plasma, tissue/cell lysate and culture supernatant samples: GM-CSF, IFNgamma, KC-like, IP-10, IL-2, IL-6, IL-7, IL-8, IL-10, IL-15, IL-18, MCP-1, and TNF-alpha. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate. The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume. Panel Type: Cytokines/Chemokines
Application:
•Analytes: GM-CSF, IFNgamma, IP-10, IL-2, IL-6, IL-7, IL-8, IL-10, IL-15, IL-18, KC-like, MCP-1, and TNF-alpha.
•Recommended Sample Type: Canine serum, plasma, cell culture supernatant and tissue/cell lysates
•Recommended Sample Dilution: 25 muL of a 1:3 dilution of plasma or serum, or 25 muL cell culture supernatant
•Assay Run Time: Overnight (16-18 hours) at 2-8C or 2 hours at room temperature (20-25 C)
•Research Category: Inflammation & Immunology
Legal Information: xMAP is a registered trademark of Luminex Corp