Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, we recommend producing self-inactivating replication incompetent viral particles in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The CMV-TagRFP Control Vector is provided as 10 mug of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/mul.
Synonyms: MISSION(R) Control Vectors
MDL Number: MFCD07785395
Storage: -20C
Application: To see more application data, protocols, vector maps visit sigma.com/shrna.The MISSION TagRFP Control Vector is an 8594 base pair lentivirus plasmid vector that contains a gene encoding TagRFP, under the control of the CMV promoter. The CMV-TagRFP Control Vector is useful as a positive control in experiments using the MISSION shRNA library clones.
Legal Information: Use of this product is subject to one or more license agreements. For details, please see issionlicense">http://sigmaaldrich.com/missionlicense.