Monoclonal Anti-ß-Catenin (mouse IgG1 isotype) is derived from the 15B8 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. ß-catenin belongs to the armadillo family and comprises the N-terminal region with 12 imperfect repeats and a conserved C-terminal region. The locus 3p22.1 in the human chromosome harbors ß-catenin gene. ß-catenin shares 70% sequence identity to a protein encoded by Drosophila armadillo, a segment polarity gene. Both armadillo and ß-catenin share considerable homology with plakoglobin, which has been proposed to be ?-catenin.
Synonyms: Monoclonal Anti-beta-Catenin
MDL Number: MFCD00282178
Storage: -20C
Application: Monoclonal Anti-ß-Catenin antibody produced in mouse has been used:
•immunoblotting
•immunohistochemistry
•immunolabeling
•immunofluorescence
•immunocytochemical staining
Biochem Physiol Actions: ß-catenin is a prime component of the wingless (Wnt) pathway and plays a key role in gene expression during development. The homology between ß-catenin and armadillo raised the possibility that ß-catenin has a developmental signaling role in vertebrates. ß-catenin mediates the interaction of the E-cadherin-catenin complex with the epidermal growth factor (EGF) receptor. ß-catenin and plakoglobin are substrates for tyrosine phosphorylation following EGF stimulation of cells. ß-catenin also associates with the tumor suppressor protein adenomatous polyposis coli (APC). Monoclonal antibody reacting specifically with ß-catenin is an essential tool in defining the interactions and distributions of ß-catenin and its relationships with other catenins and cadherins in various cells and tissues.