Synonym(s): 4-Amino-10-methylfolic acid hydrate; L-4-Amino-N10-methylpteroylglutamic acid hydrate; L-Amethopterin hydrate; Antifolan hydrate; MTX hydrate; Methylaminopterin hydrate
Formula: C20H22N8O5 · xH2O
Formula Weight: 454.44 (anhydrous basis)
CAS No.: 133073-73-1
UN Number: UN1544
Purity: ≥98% (HPLC)
Application: Potent inhibitor of dihydrofolate reductase and agent for antitumor studies.2, Use to inhibit dihydrofolate reductase in DHFR-based protein expression systems. Also effective in treatment of pyrimethamine-resistant Plasmodium vivax malaria parasites.
Storage: −20°C
Biochem/physiol Actions: Methotrexate used to inhibit dihyrofolate reductase and select for DHFR overexpresssion.
Empirical Formula (Hill Notation): C7H9N