Melting Point: 54 °C ((NaCl, 0.1 M, pH 7.0))
Storage: −20°C
UNSPSC Code: 12352200
RIDADR: NONH for all modes of transport
Features and Benefits:
Contents
Lyophilizate, sodium salt, base ratio 1:1, alternating base sequence
Application:
Poly[d(I-C)] is used as a template for RNA polymerases, and as a DNA model for biophysical investigations.
It is also used as a primer template with Kornberg DNA polymerase for the determination of small amounts of dITP, dGTP, and dCTP. Poly(dI-dC) has been used in electrophoretic mobility shift assays (EMSA).
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Quality:
Typical analysis:
Absorbance determination A250/A260, A280/A260, A290/A260
Preparation Note:
Activator: Polymines (spermine, spermidine)
Storage conditions (working solution): Solution is stable to at least 12 months if stored at 2 to 8 °C, contact with DNase or bacteria is to avoid.
Sequence:
In an aqueous solution the DNA itself is a salt (the acid groups of the phosphate back bone have a negative charge and are saturated by positive sodium ions) i.e. there is no additional salt in solution. The structural formula which shows the negative 0-groups in the phosphate backbone is available. The same number of positive sodium ions must be dissociated in solution as counter ions.
As the sodium ions are part of the product and saturate the phosphate groups, the lyophilisate is salt-free.