This product is a panel of three (3) genetically modified cell lines involving the CD19 gene. The panel consists of a CD19 knockout cell line (CD19-/-) as well as a CD19low and a CD19high expression cell line. The CD19-/- cell line was generated using CompoZr(R) Zinc Finger Nuclease technology, and the two CD19 expression cell lines were generated using MISSION(R) lentivirus. The CD19low and CD19high cell lines express CD19 at low and high levels relative to the CD19-/- cell line (CD19high > CD19low > CD19-/-) as measured via fluorescence-activated cell sorting (FACS). As a comparison, wild type Raji cells demonstrate CD19medium expression as measured via FACS. The STR profile of these cell lines match that of its parental cell line - ECACC catalog number 85011429.
Storage: -196C
Legal Information: MISSION is a registered trademark of Sigma-Aldrich Co. LLC